CD3 Antibody – 159Tb
Catalog: 715901 Clone: D7A6E Reactivity: Human*
Storage: CD3ɛ antibody is supplied in protein-based stabilizer with 0.05% sodium azide. Store at 4°C
Storage: CD3ɛ antibody is supplied in protein-based stabilizer with 0.05% sodium azide. Store at 4°C
IHC: CD3ɛ antibody staining of FFPE
human tonsil
human tonsil
MIBI: CD3ɛ antibody staining (cyan) of FFPE human tonsil, counterstained with dsDNA (magenta)
IHC: CD3ɛ antibody staining of FFPE
human thymus
human thymus
MIBI: CD3ɛ antibody staining (cyan) of FFPE human thymus; for clarity dsDNA is not shown
Background: CD3 is found on all mature T cells, serving important roles for T cell development. CD3 is part of the T cell receptor that recognizes antigen, resulting in the activation of signaling pathways. During T cell development the intensity of the response to antigen is tightly controlled to select for responsive T cells that are not autoreactive. In disease, the lack of CD3 expression is associated with a severe combined immunodeficiency (SCID) characterized by impairment of both humoral and cell-mediated immunity.
Validation: Each lot of conjugated CD3 antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL of CD3 antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results,
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
References
- Guy, C. S.
and Vignali, D. A. Organization of proximal signal initiation at the TCR: CD3 complex. Immunological
Reviews. 2009; 232: 7-21.
* Conjugate tested on human tissue.