CD68 (D4B9C) – 156Gd
Catalog: 715601 Clone: D4B9C Isotype: Rabbit IgG
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C
IHC: CD68 staining of FFPE human tonsil
MIBI: CD68 staining (cyan) of FFPE human tonsil, counterstained with histone H3 (magenta)
IHC: CD68 staining of FPE human liver
MIBI: CD68 staining (cyan) of FFPE human liver, counterstained with histone H3 (magenta)
Background: CD68 is a marker predominantly expressed on monocytes and macrophages and is used to identify tumor-associated macrophages (TAMs). Macrophages home to specific targets in part through the interaction between CD68 and tissue- and organ-specific lectins or selectins. CD68 staining can be combined with HLA-DR to identify M1 macrophages or with CD163 to identify M2 macrophages, macrophage phenotypes with prognostic associations to outcomes in certain cancers.
Validation: Each lot of conjugated antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application. Suggested starting range is 1:100.
- Mei, J. et al. Prognostic impact of tumor-associated macrophage infiltration in non-small cell lung cancer: A systemic review and meta-analysis. Oncotarget. 2016; 7(23): 34217-28.
* Conjugate tested on human tissue.