GATA3 Antibody [EPR16651] – 145Nd
Reactivity: Human*, mouse
IHC: GATA3 staining of FFPE human T-cell lymphoma
MIBI: GATA3 staining (cyan) of FFPE human T-cell lymphoma, costained with dsDNA (magenta)
IHC: GATA3 staining of FFPE human tonsil
MIBI: GATA3 staining (cyan) of FFPE human tonsil, costained with dsDNA (magenta)
Background: GATA-3 is a critical regulator of development of various systems in both mouse and human. The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells. It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system. Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome.
Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views. These results are pathologist verified.
Recommended Usage: Human FFPE: 1:100 dilution.
For optimal results, the antibody should be titrated for each desired application.
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
I-Cheng Ho et al. GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 2009 Feb;9(2):125-35.
Jonathan Chou et al. GATA3 in development and cancer differentiation: cells GATA have it! J Cell Physiol. 2010 Jan;222(1):42-9.
* Conjugate tested on human tissue.