Granzyme B Antibody – 150Nd

Catalog: 715003
Clone: EPR22645-206
Isotype: Rabbit IgG
Reactivity: Mouse* and Human
Application: MIBI-FFPE
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C

GranzymeB-IHC-staining-FFPE-mouse-spleen
IHC: Granzyme B staining of FFPE mouse spleen
GranzymeB-Ionpath-MIBI-staining-FFPE-mouse-spleen
MIBI: Granzyme B staining (cyan) of FFPE mouse spleen, costained with dsDNA (magenta)
Background: Granzyme B, one of 5 granzymes in humans, is a serine protease primarily produced by cytotoxic T cells and NK cells and released along with perforin to induce apoptosis of target cells. It serves as one of the activation markers for CD8+ memory T cells. Inflammation can also lead to granzyme B production by T helper cells, regulatory T cells, and many cell types of the myeloid lineage.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis of stained tissue microarray using the appropriate positive and negative tissue field of views. These results are pathologist verified.

Recommended Usage: Mouse FFPE: 1:100 dilution.
For optimal results, the antibody should be titrated for each desired application.

MIBI technology: Learn more about MIBI Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References
Boivin, W.A., Cooper, D.M., Hiebert, P.R., Granville, D.J. Intracellular versus extracellular granzyme B in immunity and disease: challenging the dogma. Laboratory Investigation. 2009; 89:1195–1220.
Tobias M. Nowacki et al. Granzyme B production distinguishes recently activated CD8+ memory cells from resting memory cells. Cell Immunol. 2007 May; 247(1): 36–48.

* Conjugate tested on mouse FFPE tissue.

Contact Us

Let us know how we can help you.


    Immuno-oncology/CancerNeuroscienceInfectious DiseaseImmunologyStem CellOther

    255

    X
    Contact Us

    We are using cookies on our website

    Please confirm, if you accept our tracking cookies. You can also decline the tracking and continue to visit our website without any data sent to third-party services.