Histone H3 (D1H2) – 115In

Catalog: 711501                                            Clone: D1H2                                          Isotype: Rabbit IgG
Reactivity: Human*, Mouse, Rat, Monkey
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C

IHC: Histone H3 staining of  FFPE human tonsil

MIBI: Histone H3 staining  (cyan) of FFPE human tonsil

IHC: Histone H3 staining of  FFPE human liver

MIBI: Histone H3 staining  (cyan) of FFPE human liver

Background: Found in all eukaryotic cells, histones are basic nuclear proteins important for chromatin structure and transcription regulation.  DNA is wrapped around histones into repeating units called nucleosomes.  The accessibility of specific regions of DNA to transcription factors and RNA polymerase II is tightly regulated via post-translational modifications to histones.  Histone  H3  becomes phosphorylated at multiple sites (serine 10 and 28, threonine 11) during mitosis and meiosis.

Validation: Each lot of conjugated antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

References

  • Li, Bing et al. The role of chromatin during transcription. Cell. 2007; 128(4):707 – 719.

* Conjugate tested on human tissue.

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