HLA Class I A, B and C (EMR8-5) – 160Gd
Catalog: 716001 Clone: EMR8-5 Isotype: Mouse IgG1
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C
IHC: HLA Class I staining of FFPE human tonsil
MIBI: HLA Class I staining (cyan) of FFPE human tonsil, counterstained with dsDNA (magenta)
IHC: HLA Class I staining of FFPE human liver
MIBI: HLA Class I staining (cyan) of FFPE human liver, counterstained with dsDNA (magenta)
Background: Human leukocyte antigen (HLA) Class I A, B, and C are MHC class I proteins that present peptides to cytotoxic T cells and are expressed on all nucleated cells of vertebrates. The peptides are generated by the proteasome from intracellular proteins. In healthy cells, these peptides will consist of self-proteins but from infected cells, some peptides will be from foreign proteins causing antigen-specific cytotoxic T cells to become activated and kill the cell. Certain viruses and tumors can downregulate HLA expression, avoiding detection by cytotoxic T cells that could recognize peptides from viral proteins or mutated proteins of cancer cells.
Validation: Each lot of conjugated antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application. Suggested starting range is 1:100.
- Coulie, P.G. et al. Tumour antigens recognized by T lymphocytes: at the core of cancer immunotherapy. Nat Rev Cancer. 2014; 14(2):135-46.
- Antoniou AN, Powis SJ, Elliott T. Assembly and export of MHC class I peptide ligands. Curr Opin Immunol. 2003; 15:75–81. .
* Conjugate tested on human tissue.