HLA Class I A, B and C (EMR8-5) – 160Gd

Catalog: 716001                                            Clone: EMR8-5                                          Isotype: Mouse IgG1
Reactivity: Human*
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C

IHC: HLA Class I staining of  FFPE human tonsil

MIBI: HLA Class I staining  (cyan) of FFPE human tonsil, counterstained with dsDNA (magenta)

IHC: HLA Class I staining of  FFPE human liver

MIBI: HLA Class I staining  (cyan) of FFPE human liver, counterstained with dsDNA (magenta)

Background: Human leukocyte antigen (HLA) Class I A, B, and C are MHC class I proteins that present peptides to cytotoxic T cells and are expressed on all nucleated cells of vertebrates.  The peptides are generated by the proteasome from intracellular proteins. In healthy cells, these peptides will consist of self-proteins but from infected cells, some peptides will be from foreign proteins causing antigen-specific cytotoxic T cells to become activated and kill the cell. Certain viruses and tumors can downregulate HLA  expression,  avoiding  detection by cytotoxic T  cells  that could recognize peptides from viral proteins or mutated proteins of cancer cells.

Validation: Each lot of conjugated antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

References

  • Coulie, P.G. et al. Tumour antigens recognized by T lymphocytes: at the core of cancer immunotherapy. Nat Rev  Cancer. 2014; 14(2):135-46.
  • Antoniou AN, Powis SJ, Elliott T. Assembly and export of MHC class I peptide ligands. Curr Opin Immunol. 2003;  15:75–81. .

* Conjugate tested on human tissue.

Contact Us

We are glad that you prefer to contact us. Please fill our short form and one of our friendly team members will contact you back.


Immuno-oncology/CancerNeuroscienceInfectious DiseaseImmunologyStem CellOther

X
Contact Us

We are using cookies on our website

Please confirm, if you accept our tracking cookies. You can also decline the tracking, so you can continue to visit our website without any data sent to third party services.