LAG3 Antibody – 147Sm

Catalog: 714701                                        Clone: 17B4                                         Isotype: Mouse IgG
Reactivity: Human*
Storage: LAG3 antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C
IHC: LAG3 staining of FFPE human tonsil
MIBI: LAG3 staining (cyan) of FFPE human tonsil, costained with dsDNA (magenta)
IHC: LAG3 staining of FFPE human melanoma

MIBI: LAG3 staining (cyan) of FFPE human melanoma, costained with dsDNA (magenta)

Background: LAG3 (lymphocyte activation gene-3, CD223) is structurally similar to CD4 and is expressed on activated T cells, regulatory T cells, NK cells, B cells and plasmacytoid dendritic cells. LAG3 is an immune checkpoint receptor, binding to MHC-II with greater affinity than CD4 and inhibiting cell activation and proliferation.  A second ligand of LAG3 is fibrinogen-like protein 1 (FGL1), a liver-secreted protein that is also expressed by tumor cells contributing to tumor evasion of the immune system.

Validation: Each lot of conjugated LAG3 antibody is quality control tested by MIBIscope™ analysis of stained tissue microarrays using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL of LAG3 antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

MIBI technology: Learn more about MIBI Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References

  • Wang et al., Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3, Cell (2019),
    https:// doi.org/10.1016/j.cell.2018.11.010.

 

* Conjugate tested on human tissue.

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