MIBIscope™ I

First Multiplexed Ion Beam Imaging (MIBI) System

MIBIscope™ I provides scientists with new insights in the understanding of immune cell populations within the tumor microenvironment (TME).  Accurate and quantitative data is obtained using Secondary Ion Mass Spectrometry (SIMS), a highly sensitive and robust mass spectrometry imaging method.

Leading biopharma and biomedical research institutes are currently piloting MIBIscope™ I in research applications including immune-oncology, immunology and neuroscience where high-fidelity multiplexed imaging data is needed to make new discoveries.

 

Multiplexed Tissue Imaging with Publication-Quality Data

The MIBIscope™ I workflow supports simultaneous staining, detection, and visualization of 40+ markers. The instrument’s large dynamic range enables both high and low abundance proteins to be captured and visualized simultaneously.

With MIBItracker software, scientists can easily review image quality following a run, assess expression profiles across multiple cells, and evaluate immune cell populations through the convenience of a web browser. Images can also be exported as TIFF files to readily insert into publications, or for subsequent analysis in third-party packages such as Fiji, HALO®, VisioPharm®, and QuPath.

IHC Compatible Workflow Enables Easy Integration into Pathology Laboratories

MIBIscope™ I does not require special ventilation. This enables easy installation and lab setup while minimizing costs associated with modifying laboratory infrastructure.

Sample preparation follows conventional IHC protocols and MIBIscope™ I is compatible with fresh, frozen and FFPE tissue types.  As the MIBIscope I does not ablate the entire sample, precious tissue is conserved and may be scanned repeatedly.

 

Designed for the Reproducible and Reliable Results Required in Translational Research

MIBIscope™ I is designed for clinical research use and our evaluations include multiple tissue microarray (TMA) runs using FFPE tissue to demonstrate both run-to-run and intra-run reproducibility.

 

Please complete the email preferences form to be informed of the commercial launch of MIBIscope™ I.

 

Regression Analysis Comparing Full Panel vs Leave-One-Out

Generating robust multiparametric data requires controlling or minimizing the source of data variability including noise that can arise from sample processing and instrument performance. In this study, 16 different recuts from the same tissue sample were stained with either the full panel or one of 15 panels lacking one antibody (Leave-One-Out). Regression analysis comparing the signal of the markers present between the full panel and a leave-one-out panel show repeatable signal between the different sections and acquired from different runs.

Three key points are demonstrated by this experiment:

  1. The instrument performance within and between runs is robust.
  2. Sample processing is repeatable.
  3. Channel cross talk is minimal.

Month to month reproducibility

Images of tonsil sections stained at the same time and acquired by MIBI™ platform one month apart show repeatable detection of immune subsets including PD1+ T cells.

Month 1. CD3 (blue), CD8 (green), CD68 (red).

Month 2. CD3 (blue), CD8 (green), CD68 (red).

Month 1. PD-L1 (yellow), PD1 (cyan).

Month 2. PD-L1 (yellow), PD1 (cyan).

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Immuno-oncology/CancerNeuroscienceInfectious DiseaseImmunologyStem CellOther

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