PD-L1 (E1L3N) – 149Sm

Catalog: 714901                                            Clone: E1L3N                                            Isotype: Rabbit IgG
Reactivity: Human*
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C

IHC: PD-L1 staining of FFPE human placenta

MIBI: PD-L1 staining (cyan) of FFPE human placenta, counterstained with dsDNA (magenta)

IHC: PD-L1 staining of FFPE human tonsil

MIBI: PD-L1 staining (cyan) of FFPE human tonsil, counterstained with dsDNA (magenta)

Background: Programmed cell death 1 ligand 1 (PD-L1, CD274) binds to PD-1 and inhibits T cell activation. APCs, activated T cells, and tissues including placenta, heart, and lung can express PD-L1. PD-L1 is expressed in cancer as a means for cancerous cells to inhibit immune responses. PD-L1 has been detected for several tumor types including melanoma, lung, ovary, colon, breast, and renal cell carcinomas. PD-L1 expression in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma. Inhibition of the PD-1-PD-L1 axis has been an active area of clinical research with several approved drugs for multiple indications.

Validation: Each lot of conjugated antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

References

  • Wimberly, H. et al. PD-L1 Expression Correlates with Tumor-Infiltrating Lymphocytes and Response to Neoadjuvant Chemotherapy in Breast Cancer. Cancer Immunol Res. 2015; 3(4): 326-332.
  • Bindels, S. et al. Regulation of PD-L1 by SIP1 in human epithelial breast tumor cells. Oncogene. 2006; 25:4975–4985.

* Conjugate tested on human tissue.

Contact Us

We are glad that you prefer to contact us. Please fill our short form and one of our friendly team members will contact you back.


Immuno-oncology/CancerNeuroscienceInfectious DiseaseImmunologyStem CellOther

X
Contact Us

We are using cookies on our website

Please confirm, if you accept our tracking cookies. You can also decline the tracking, so you can continue to visit our website without any data sent to third party services.