Vimentin (D21H3) – 163Dy

Catalog: 716301                                          Clone: D21H3                                       Isotype: Rabbit IgG
Reactivity: Human*
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C

IHC: Vimentin staining of  FFPE human tonsil

MIBI: Vimentin staining
(cyan) of FFPE human tonsil,  counterstained with dsDNA  (magenta)

IHC: Vimentin staining of  FFPE human liver

MIBI: Vimentin staining
(cyan) of FFPE human liver,  counterstained with  dsDNA (magenta)

Background: The cytoskeleton is composed of microfilaments, intermediate filaments, and microtubules. Vimentin is a class-III  intermediate  filament of mesenchyme origin  (fibroblasts,  meothelium,  endothelium,  adipocytes, myoblasts, chondroblasts, osteoblasts). Vimentin is phosphorylated at Ser-55 during mitosis, among other sites, and vimentin undergoes reorganization during this time.  Vimentin expression in tumour epithelial cells has been reported as negative prognostic indicator for survival for patients with lung cancer and hepatocellular and breast carcinoma. High expression of vimentin is thought to contribute to the migratory and invasive phenotype of metastatic cells.

Validation: Each lot of conjugated antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

References

  • Al-Saad, S. et al. The prognostic impact of NF-𝛋B p105, vimentin, E-cadherin and Par6 expression in epithelial and stromal compartment in non-small-cell lung cancer. Br J Cancer. 2008 Nov 4; 99(9): 1476–1483.
    Bindels, S. et al. Regulation of vimentin by SIP1 in human epithelial breast tumor cells. Oncogene.
    2006;25:4975–4985.

* Conjugate tested on human tissue.

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