CD20 Antibody – 167Er
Catalog: 716701 Clone: L26 Isotype: Mouse IgG2a
Reactivity: Human*
Storage: CD20 antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C
IHC: CD20 staining of FFPE human tonsil
MIBI: CD20 staining (cyan) of FFPE human tonsil, costained with dsDNA (magenta)
IHC: CD20 staining of FFPE human thymus
MIBI: CD20 staining (cyan) of FFPE human thymus, costained with dsDNA (magenta)
Background: CD20 is an activated-glycosylated phosphoprotein expressed on B cells from late pro-B cells through memory B cells but is absent from plasma cells. CD20 is involved in the transport of calcium and calcium-dependent intracellular signaling pathways. Anti-CD20 therapy is used to treat diseases with a B cell component such as B-cell chronic lymphocytic leukemia (B-CLL) and rheumatoid arthritis (RA).
Validation: Each lot of conjugated CD20 antibody is quality control tested by MIBIscope™ analysis of stained tissue microarrays using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL of CD20 antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application. Suggested starting range is 1:100.
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
References
- Walport M, Murphy K, Janeway C, Travers PJ (2008). Janeway’s Immunobiology (7th ed.). New York: Garland Science.
- Payandeh, Z., Bahrami, A. A., Hoseinpoor, R., Mortazavi, Y., Rajabibazl, M., Rahimpour, A., Taromchi, A. H., Khalil, S. The applications of anti-CD20 antibodies to treat various B cells disorders. Biomedicine & Pharmacotherapy. 2019; 109:2415-2426.
* Conjugate tested on human tissue.