MIBI™ technology (Multiplexed Ion Beam Imaging) uses Secondary-Ion Mass Spectrometry (SIMS), a type of mass spectrometry traditionally used in the semiconductor industry, to image antibodies tagged with monoisotopic metal reporters.
This unique technology enables:
- Visualization of 40+ markers simultaneously
- Imaging at the sub-cellular resolution
- Detection of low abundance proteins
- Rescanning of slides at multiple resolutions
The system uses a simple, streamlined workflow that adapts easily for both pathology and clinical imaging labs. Samples are prepared using a traditional IHC protocol and data are collected in less than 1 hour using the MIBIscope™ I.
- PREPARE: FFPE tissue preparation follows conventional IHC protocols.
- STAIN: Tissue is stained with a mixture of validated antibodies with conjugated elemental reporters, in one single step.
- DETECT: Stationary tissue samples are raster-scanned with an ion beam and secondary elemental ions are generated.
- IMAGE: All markers are imaged at the same time and detected via TOF mass spectrometry
Highly Multiplexed Tissue Imaging
In a single scan, the MIBIscope™ I can measure expression of 40+ targets including both high and low abundance proteins at light microscopy resolution.
Low abundance proteins such as PD-1 and FOXP3 that can be missed using other techniques are easily detected with the MIBIscope™ I. With no probe background or autofluorescence to interfere with weak signals, the high sensitivity that results ensures all high, medium and low-abundance targets are detected in the same scan.
True Sub-Cellular Resolution
With resolution comparable to light microscopy systems, MIBIscope™ I system lets you analyze and interpret cellular interactions. Cellular structures as small as 250 nm can be imaged, enabling downstream cell segmentation data analysis. Because the MIBIscope™ I doesn’t fully ablate the sample, a survey scan can be done to select specific regions of interest followed by high-resolution imaging.
Rescan Slides at Multiple Resolutions
The system’s imaging workflow enables scientists and pathologists to scan a whole tissue section faster at low resolution, then rescan regions of interest at high resolution. Precious tissue is conserved and may be scanned repetitively.
Detection of keratin (orange), CD3 (blue), CD8 (green) and CD68 (red) in lung cancer sample.
Detection of keratin (orange), CD3 (blue), CD68 (red), PD-1 (yellow) and PD-L1 (cyan) in placenta.
Detection of keratin (orange), CD3 (blue), CD8 (green) and CD68 (red) in squamous cell carcinoma (head and neck).