Reagents
Overview of the MIBI staining protocol with Ionpath reagents and slides.

Prepare and Bake the Slides
Ionpath Reagent:
- MIBI Slides (Cat #: 567001)


Deparaffinization and Rehydration
Ionpath Reagent:
- MIBI diH2O (Cat#: 567002)


Heat-Induced Antigen Retrieval


Wash
Ionpath Reagent:
- MIBI 20X TBS-T (Cat#: 567005)


Blocking


Antibody Staining
Ionpath Reagents:
- MIBI Conjugated Antibodies (Cat#: variable)
OR
- MIBI Conjugation Kits (Cat#: variable)


Fixation and Dehydration
Ionpath Reagents:
- MIBI 20X TBS-T (Cat#: 567005)
- MIBI 10X PBS (Cat#: 567004)
- MIBI 10X Tris pH8.5 (Cat#: 567003)
- MIBI diH2O (Cat#: 567002)

Dry and Store the Slides