Reagents
Overview of the MIBI staining protocol with Ionpath reagents and slides.
![Bake Bake](https://www.ionpath.com/wp-content/uploads/2018/09/Bake.png)
Prepare and Bake the Slides
Ionpath Reagent:
- MIBI Slides (Cat #: 567001)
![arrow grey arrow grey](https://www.ionpath.com/wp-content/uploads/2018/09/arrow-grey-50-opacity.png)
![slide slide](https://www.ionpath.com/wp-content/uploads/2018/09/slide.png)
Deparaffinization and Rehydration
Ionpath Reagent:
- MIBI diH2O (Cat#: 567002)
![arrow grey arrow grey](https://www.ionpath.com/wp-content/uploads/2018/09/arrow-grey-50-opacity.png)
![Heat induced antigen retrieval Heat induced antigen retrieval](https://www.ionpath.com/wp-content/uploads/2018/09/Heat-induced-antigen-retrieval.png)
Heat-Induced Antigen Retrieval
![arrow grey arrow grey](https://www.ionpath.com/wp-content/uploads/2018/09/arrow-grey-50-opacity.png)
![wash wash](https://www.ionpath.com/wp-content/uploads/2018/09/wash.png)
Wash
Ionpath Reagent:
- MIBI 20X TBS-T (Cat#: 567005)
![arrow grey arrow grey](https://www.ionpath.com/wp-content/uploads/2018/09/arrow-grey-50-opacity.png)
![IMG-MIBI-blocking-Artboard 3-1X MIBI antibody blocking](https://www.ionpath.com/wp-content/uploads/2020/12/IMG-MIBI-blocking-Artboard-3-1X.png)
Blocking
![arrow grey arrow grey](https://www.ionpath.com/wp-content/uploads/2018/09/arrow-grey-50-opacity.png)
![IMG-MIBI-staining-Artboard 4-1X MIBI antibody staining](https://www.ionpath.com/wp-content/uploads/2020/12/IMG-MIBI-staining-Artboard-4-1X.png)
Antibody Staining
Ionpath Reagents:
- MIBI Conjugated Antibodies (Cat#: variable)
OR
- MIBI Conjugation Kits (Cat#: variable)
![arrow grey arrow grey](https://www.ionpath.com/wp-content/uploads/2018/09/arrow-grey-50-opacity.png)
![wash wash](https://www.ionpath.com/wp-content/uploads/2018/09/wash.png)
Fixation and Dehydration
Ionpath Reagents:
- MIBI 20X TBS-T (Cat#: 567005)
- MIBI 10X PBS (Cat#: 567004)
- MIBI 10X Tris pH8.5 (Cat#: 567003)
- MIBI diH2O (Cat#: 567002)
![arrow grey arrow grey](https://www.ionpath.com/wp-content/uploads/2018/09/arrow-grey-50-opacity.png)
Dry and Store the Slides