Comprehensive Enumeration of Immune Cells in Solid Tumors
Webinar presented by Mike Angelo, MD. Ph.D, Pincipal Investigator, The Stanford Blood Center
Abstract: Understanding the role of distinct cellular phenotypes in tissue function, development, and pathogenesis requires tools that can rapidly and consistently quantify the expression of multiple proteins while preserving spatial information. Multiplexed Ion Beam Imaging by Time-Of-Flight (MIBI-TOF) detects elements from hydrogen to uranium, permitting simultaneous measurement of up to 42 labeled antibodies along with histochemical stains and native biological elements. We recently used this capability to analyze infiltrating immune cell populations in archival formalin-fixed paraffin embedded (FFPE) tissue sections from 42 triple negative breast cancer patients. Spatial enrichment analysis showed immune mixed and compartmentalized tumors, coinciding with expression of PD1, PD-L1 and IDO in a cell-type and location-specific manner. Ordered immune structures along the tumor-immune border served as a hallmark of compartmentalization and were linked to survival. We are currently working to build upon this initial effort to develop scoring mechanisms for guiding immune therapy drug selection.
- Learn how MIBI technology enables simultaneous imaging of 40+ markers in FFPE tissue.
- Learn how tumor expression and immune composition are interrelated with histological context that correlates with overall survival in TNBC
Comprehensive Capture of Human Neuropathology by Multiplexed Ion Beam Imaging (MIBI)
Webinar presented by Sean Bendall, MD. Ph.D, Pincipal Investigator, The Stanford Blood Center
Abstract: Single cell analysis, starting with the earliest low parameter fluorescent experiments, helped define the major cell subsets of human cellular systems as we understand them today. Now, a novel combination of single cell analysis and metal isotopes based mass spectrometry (MIBI) offers a routine examination of 30+ parameters at the nanometer scale, without interference of spectral overlap characteristic of fluorescent reporters. With this platform, we have reached new levels of organizational understanding in human pathobiology – especially when combined with novel single-cell visualization and analysis methods.
Neurodegenerative related disease is estimated to affect five million or more of the ageing American population by 2050. Cognitive care for these individuals is estimated to surpass a trillion dollars a year by the middle of next decade. Accordingly, there is an immediate need for new therapeutic strategies to address this along with identifying unique predictive signatures that correlate with disease mechanism.
During this webinar, we will highlight early applications of this new imaging technology to resolve human neurodegenerative disease and cognitive resilience in retrospective studies of human CNS tissues. This work begins to reveal unappreciated layers of human cellular organization and structure in human systems that can be exploited to understand and perturb human pathobiology.
- Learn the challenges and opportunities for imaging archival CNS tissue
- Learn the principles of Multiplex Ion Beam Imaging (MIBI)
- Learn how MIBI is used to capture multi-scale observations in human neuropathology