A structured tumor-immune microenvironment in triple negative breast cancer revealed by multiplexed ion beam imaging
Keren et al. Cell, Volume 174, Issue 6, P1373-1387. E19. September 06, 2018
Tissue imaging by mass spectrometry: a practical guide for the medicinal chemist
Abstract: Understanding the tissue distribution of therapeutic molecules is often critical for assessing their efficacy and toxicity. Unfortunately, standard methods for monitoring localized drug distribution are resource-intensive and are typically performed late in the discovery process. As a result, early development efforts often progress without detailed information on the effect that changes in structure and/or formulation have on drug localization. Recent innovations in mass spectrometry (MS) provide new options for mapping the spatial distribution of
Johnson et al. ACS Medical Chemistry Letters.
Multiplexed ion beam imaging analysis for quantitation of protein expression in cancer tissue sections
Rost et al. LAb Invest. 2017 Aug;97(8):992-1003
Multiplexed ion beam imaging of human breast tumors
tagged with isotopically pure elemental metal reporters. Multiplexed ion beam imaging (MIBI) is capable of analyzing up to 100 targets simultaneously over a five-log dynamic range. Here, we used MIBI to analyze formalin-fixed,
paraffin-embedded human breast tumor tissue sections stained with ten labels simultaneously. The resulting data suggest that MIBI can provide new insights into disease pathogenesis that will be valuable for basic research,
drug discovery and clinical diagnostics.
Angelo et al. Nature Medecine 2014 Apr;20(4):436-42.
SITC 2018 – P106
Multiplexed Ion Beam Imaging For Characterization of the Tumor Microenvironment Across Tumor Types
SITC 2018 – P20
A Structured Tumor Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Imaging