First Multiplexed Ion Beam Imaging (MIBI™) System
MIBIscope™ provides scientists with new insights in the understanding of immune cell populations within the tumor microenvironment (TME). Accurate and quantitative data is obtained using Secondary Ion Mass Spectrometry (SIMS), a highly sensitive and robust mass spectrometry imaging method.
Leading biopharma and biomedical research institutes are currently piloting MIBIscope in research applications including immuno-oncology, immunology, and neuroscience where high-fidelity multiplexed imaging data is needed to make new discoveries.
Multiplexed Tissue Imaging with Publication-Quality Data
The MIBIscope workflow supports simultaneous staining, detection, and visualization of 40+ markers. The instrument’s large dynamic range enables both high and low abundance proteins to be captured and visualized simultaneously.
With MIBItracker software, scientists can easily review image quality following a run, assess expression profiles across multiple cells, and evaluate immune cell populations through the convenience of a web browser. Images can also be exported as TIFF files to readily insert into publications, or for subsequent analysis in third-party packages such as Fiji, HALO®, VisioPharm®, and QuPath.
IHC Compatible Workflow Enables Easy Integration into Pathology Laboratories
MIBIscope does not require special ventilation. This enables easy installation and lab setup while minimizing costs associated with modifying laboratory infrastructure.
Sample preparation follows conventional IHC protocols and MIBIscope is compatible with fresh, frozen, and FFPE tissue types. As the MIBIscope does not ablate the entire sample, precious tissue is conserved and may be scanned repeatedly.
Designed for the Reproducible and Reliable Results Required in Translational Research
MIBIscope™ is designed for clinical research use and our evaluations include multiple tissue microarray (TMA) runs using FFPE tissue to demonstrate both run-to-run and intra-run reproducibility.
Regression Analysis Comparing Full Panel vs Leave-One-Out
- The instrument performance within and between runs is robust.
- Sample processing is repeatable.
- Channel cross talk is minimal.
Month-to-month reproducibilityImages of tonsil sections stained at the same time and acquired by the MIBI™ platform one month apart show repeatable detection of immune subsets including PD1+ T cells.
Month 1. CD3 (blue), CD8 (green), CD68 (red).
Month 2. CD3 (blue), CD8 (green), CD68 (red).
Month 1. PD-L1 (yellow), PD1 (cyan).
Month 2. PD-L1 (yellow), PD1 (cyan).