Background: CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), plays roles in leukocyte transmigration, angiogenesis, and integrin activation. Specifically, CD31 is highly expressed at endothelial cell-cell junctions, functioning as an adhesion protein to maintain endothelial cell junctional integrity and restore the vascular permeability barrier following inflammatory or thrombotic challenge. The expression of CD31 on endothelial cells makes it a useful marker of the vasculature within the tumor. CD31 can also be found on platelets, monocytes, and granulocytes.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views.

Recommended Usage: Mouse FFPE: 1 ug/mL dilution. For optimal results, the antibody should be titrated for each desired application.

References
Lertkiatmongkol P., Liao D., Mei H., Hu Y., Newman P.J. Endothelial functions of platelet/endothelial cell adhesion molecule-1 (CD31). Curr Opin Hematol. 2016; 23(3):253-9.

* Conjugate tested on mouse FFPE tissue.