CD8 Antibody – 158Gd
Catalog: 715801 Clone: C8/144B Isotype: Mouse IgG
Reactivity: Human*
Storage: CD8 antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C
Reactivity: Human*
Storage: CD8 antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C
IHC: CD8 staining of FFPE human tonsil
MIBI: CD8 staining (cyan) of FFPE human tonsil, costained with dsDNA (magenta)
IHC: CD8 staining of FFPE human thymus
MIBI: CD8 staining (cyan) of FFPE human thymus, costained with dsDNA (magenta)
Background: CD8 is a co-receptor for the T cell receptor (TCR) and is expressed on cytotoxic T cells. Cortical thymocytes, dendritic cells and NK cells can also express CD8. CD8 binds to MHC Class I to aid in antigen recognition and TCR-mediated activation. CD8 forms dimers of CD8ɑ and CD8β and clone C8/144B recognizes the alpha form of CD8.
Validation: Each lot of conjugated CD8 antibody is quality control tested by MIBIscope™ analysis of stained tissue microarrays using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL of CD8 antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application. Suggested starting range is 1:100.
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
References
- Guy, C. S. and Vignali, D. A. Organization of proximal signal initiation at the TCR:CD3 complex. Immunological Reviews. 2009; 232: 7-21.
- Gao, G. F., Rao, Z., and Bell, J. I. Molecular coordination of αβ T-cell receptors and coreceptors CD8 and CD4 in their recognition of peptide-MHC ligands, Trends in Immunology, 2002; 23(8):408-13.
* Conjugate tested on human tissue.