DC-SIGN Antibody – 173Yb
Storage: DC-SIGN antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C
IHC: DC-SIGN antibody staining of FFPE human tonsil
MIBI: DC-SIGN antibody staining (cyan) of FFPE human tonsil, costained with dsDNA (magenta)
IHC: DC-SIGN antibody staining of FFPE human liver
MIBI: DC-SIGN antibody staining
(cyan) of FFPE human liver, costained with dsDNA (magenta)
Background: DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin) also known as CD209, is expressed on myeloid dendritic cells and macrophages. DC-SIGN binds to ICAM-2, ICAM-3 and is a receptor for certain viruses including HIV-1, transmitting HIV-1 to CD4 expressing T cells. DC-SIGN has roles in CD4+ T cell activation, dendritic cell rolling and transendothelial migration.
Validation: Each lot of conjugated DC-SIGN antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL of DC-SIGN antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application. Suggested starting range is 1:100.
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
Geijtenbeek, T.B., Kwon, D.S., Torensma, R., van Vliet, S.J., van Duijnhoven, G.C., Middle, J., Cornelissen, I.L., Nottet, H.S., KewalRamani, V.N., Littman, D.R., Figdor, C.G., van Kooyk, Y. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell. 2000; 100 (5): 587–97.
* Conjugate tested on human tissue.