Histone H3 Antibody – 115In
Reactivity: Human*, Mouse, Rat, Monkey
Storage: Histone H3 antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C
IHC: Histone H3 antibody staining of FFPE human tonsil
MIBI: Histone H3 antibody staining (cyan) of FFPE human tonsil
IHC: Histone H3 antibody staining of FFPE human liver
MIBI: Histone H3 antibody staining (cyan) of FFPE human liver
Background: Found in all eukaryotic cells, histones are basic nuclear proteins important for chromatin structure and transcription regulation. DNA is wrapped around histones into repeating units called nucleosomes. The accessibility of specific regions of DNA to transcription factors and RNA polymerase II is tightly regulated via post-translational modifications to histones. Histone H3 becomes phosphorylated at multiple sites (serine 10 and 28, threonine 11) during mitosis and meiosis.
Validation: Each lot of histone H3 conjugated antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL of Histone H3 antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results,
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
- Li, Bing et al. The role of chromatin during transcription. Cell. 2007; 128(4):707 – 719.
* Conjugate tested on human tissue.