TIM-3 Antibody – 162Dv

Catalog: 716201                                        Clone: EPR22241                                         Isotype: Rabbit IgG
Reactivity: Human*
Storage: TIM-3 antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C
IHC: TIM-3 staining of FFPE human placenta
MIBI: TIM-3 staining (cyan) of FFPE human placenta, costained with dsDNA (magenta)
IHC: TIM-3 staining of FFPE human lung adenocarcinoma
MIBI: TIM-3 staining (cyan) of FFPE human lung adenocarcinoma, costained with dsDNA (magenta)
Background: TIM-3 (T-cell immunoglobulin and mucin-domain containing-3) is an immune checkpoint protein involved in negatively regulating immune responses. TIM-3 can be expressed on T cells including regulatory T cells, NK cells, monocytes and macrophages. In cancer, TIM-3 has been identified on tumor-infiltrating lymphocytes making TIM-3 an attractive target for reversing the immune suppression that is characteristic of the tumor microenvironment. Outside of cancer, TIM-3 contributes to T cell exhaustion that occurs from chronic viral infections and importantly helps mediate maternal-fetal tolerance.

Validation: Each lot of conjugated TIM-3 antibody is quality control tested by MIBIscope™ analysis of stained tissue microarrays using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL of TIM-3 antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

MIBI technology: Learn more about MIBI Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References

  • Das, M., Zhu, C., Kuchroo, V.K. Tim-3 and its role in regulating anti-tumor immunity. Immunol Rev. 2017; 276(1):97-111.
  • Li, Y. et al. Tim-3 signaling in peripheral NK cells promotes maternal-fetal immune tolerance and alleviates pregnancy loss. Sci Signal. 2017; 10, eaah4323.

* Conjugate tested on human tissue.

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