Vimentin Antibody – 163Dy

Catalog: 716301                                          Clone: D21H3                                       Isotype: Rabbit IgG
Reactivity: Human*
Storage: Vimentin antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C

IHC: Vimentin antibody staining of  FFPE human tonsil

MIBI: Vimentin antibody staining
(cyan) of FFPE human tonsil,  counterstained with dsDNA  (magenta)

IHC: Vimentin antibody staining of  FFPE human liver

MIBI: Vimentin antibody staining
(cyan) of FFPE human liver,  counterstained with  dsDNA (magenta)

Background: The cytoskeleton is composed of microfilaments, intermediate filaments, and microtubules. Vimentin is a class-III  intermediate  filament of mesenchyme origin  (fibroblasts,  meothelium,  endothelium,  adipocytes, myoblasts, chondroblasts, osteoblasts). Vimentin is phosphorylated at Ser-55 during mitosis, among other sites, and vimentin undergoes reorganization during this time.  Vimentin expression in tumour epithelial cells has been reported as negative prognostic indicator for survival for patients with lung cancer and hepatocellular and breast carcinoma. High expression of vimentin is thought to contribute to the migratory and invasive phenotype of metastatic cells.

Validation: Each lot of conjugated vimentin antibody is quality control tested by MIBIscope™ analysis of stained tissue microarray using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL of Vimentin antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

MIBI technology: Learn more about MIBI Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.


  • Al-Saad, S. et al. The prognostic impact of NF-𝛋B p105, vimentin, E-cadherin and Par6 expression in epithelial and stromal compartment in non-small-cell lung cancer. Br J Cancer. 2008 Nov 4; 99(9): 1476–1483.
    Bindels, S. et al. Regulation of vimentin by SIP1 in human epithelial breast tumor cells. Oncogene.

* Conjugate tested on human tissue.

Contact Us

We are glad that you prefer to contact us. Please fill our short form and one of our friendly team members will contact you back.

Immuno-oncology/CancerNeuroscienceInfectious DiseaseImmunologyStem CellOther


Contact Us

We are using cookies on our website

Please confirm, if you accept our tracking cookies. You can also decline the tracking and continue to visit our website without any data sent to third-party services.