Background: CD14 is highly expressed on monocytes and macrophages and at much lower levels on neutrophils and some dendritic cells. Non-classical monocytes have lower expression of CD14 than classical monocytes and express CD16. CD14 recognizes pathogen-associated molecular patterns (PAMPs) and is a coreceptor for lipopolysaccharide (LPS), expressed by Gram-negative bacteria.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis of stained tissue microarray using the appropriate positive and negative tissue field of views. 

Recommended Usage: Human FFPE: 1 ug/mL dilution.
For optimal results, the antibody should be titrated for each desired application.

MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References
Ziegler-Heitbrock, L. Blood Monocytes and Their Subsets: Established Features and Open Questions. Front Immunol. 2015; 17(6):423.

* Conjugate tested on human tissue.