CD45 Antibody – 175Lu
Catalog: 717501 Clone: 2B11 & PD7/26 Isotype: Mouse IgG1
Reactivity: Human*
Storage: CD45 antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store a 4°C
IHC: CD45 staining of FFPE human tonsil
MIBI: CD45 staining (cyan) of FFPE human tonsil, costained with dsDNA (magenta)
IHC: CD45 staining of FFPE human thymus
MIBI: CD45 staining (cyan) of FFPE human thymus, costained with dsDNA (magenta)
Background: CD45 is a tyrosine phosphatase expressed on all hematopoietic cells, except erythrocytes or platelets. CD45 regulates B cell receptor (BCR) and T cell receptor (TCR) activity by dephosphorylating kinases in these antigen/receptor pathways. The regulation of the TCR and BCR pathways are dependent upon the spatial organization of kinases and phosphatases. In the initial stage of receptor signaling, CD45 is excluded from the signaling area.
Validation: Each lot of conjugated CD45 antibody is quality control tested by MIBIscope™ analysis of stained tissue microarrays using the appropriate positive and negative tissue field of views and are pathologist verified.
Recommended Usage: 1 uL of CD45 antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application. Suggested starting range is 1:100.
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
References
- Leupin O., Zaru R., Laroche T., Müller S., Valitutti S.Exclusion of CD45 from the T-cell receptor signaling area in antigen-stimulated T lymphocytes. Curr Biol. 2000;10(5):277-80.
* Conjugate tested on human tissue.