F4/80 [D2S9R] Antibody – 156Gd
Isotype: Rabbit IgG
IHC: F4/80 staining of FFPE mouse liver
MIBI: F4/80 staining (cyan) of FFPE mouse liver, costained with dsDNA (magenta)
Background: F4/80, a murine homolog of EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1), is a 160 kD glycoprotein that, in humans, is known to be expressed on eosinophils. F4/80 is a widely used marker for murine macrophages, including peritoneal macrophages, alveolar macrophages, Kuffer cells, Langerhans cells, thymus and splenic red pulp macrophages. F4/80 has also been shown to be expressed on a small subset of murine dendritic cells.
Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis of stained tissue microarray using the appropriate positive and negative tissue field of views. These results are pathologist verified.
Recommended Usage: Mouse FFPE Tissue: 1:100 dilution.
For optimal results, the antibody should be titrated for each desired application.
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
McKnight AJ et al. (1996). Molecular cloning of F4/80, a murine macrophage-restricted cell surface glycoprotein with homology to the G-protein-linked transmembrane 7 hormone receptor family. J Biol Chem. 5;271(1):486-9.
* Conjugate tested on mouse tissue.