Podoplanin Antibody – 170Er
Catalog: 717002
Clone: D2-40
Isotype: Mouse IgG1
Reactivity: Human*
Application: MIBI-FFPE
Storage: Podoplanin antibody is supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C
IHC: Podoplanin staining of FFPE human tonsil
MIBI: Podoplanin staining (cyan) of FFPE human tonsil, costained with dsDNA (magenta)
IHC: Podoplanin staining of FFPE human lung squamous cell carcinoma
MIBI: Podoplanin staining (cyan) of FFPE human lung squamous cell carcinoma, costained with dsDNA (magenta)
Background: Podoplanin is a mucin-type membrane protein mainly expressed on the endothelium of lymphatic vessels and variably expressed in tumor cells, such as squamous cell carcinomas, and in cancer associated fibroblasts (CAFs). High levels of podoplanin are associated with an increased incidence of metastasis to lymph nodes and decreased survival time of patients.
Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views.
Recommended Usage: Human FFPE: 3 ug/mL dilution. For optimal results, the antibody should be titrated for each desired application.
MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.
References
Ugorski, M., Dziegiel, P., Suchanski, J. Podoplanin – a small glycoprotein with many faces. Am J Cancer Res. 2016; 6(2):370-86.
* Conjugate tested on human tissue.